Composite

Part:BBa_K4123001:Design

Designed by: Franco Herrera   Group: iGEM22_Manchester   (2022-10-11)


Plasmid for the synthesis of Auxin (IAA)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 501
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 1305
    Illegal EcoRI site found at 1623
    Illegal EcoRI site found at 3466
    Illegal EcoRI site found at 3627
    Illegal XbaI site found at 16
    Illegal XbaI site found at 1638
    Illegal XbaI site found at 3642
    Illegal SpeI site found at 3599
    Illegal SpeI site found at 5184
    Illegal PstI site found at 1438
    Illegal PstI site found at 3613
    Illegal PstI site found at 5198
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 1305
    Illegal EcoRI site found at 1623
    Illegal EcoRI site found at 3466
    Illegal EcoRI site found at 3627
    Illegal NheI site found at 1290
    Illegal SpeI site found at 502
    Illegal SpeI site found at 3599
    Illegal SpeI site found at 5184
    Illegal PstI site found at 516
    Illegal PstI site found at 1438
    Illegal PstI site found at 3613
    Illegal PstI site found at 5198
    Illegal NotI site found at 7
    Illegal NotI site found at 509
    Illegal NotI site found at 1629
    Illegal NotI site found at 3606
    Illegal NotI site found at 3633
    Illegal NotI site found at 5191
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 1305
    Illegal EcoRI site found at 1623
    Illegal EcoRI site found at 3466
    Illegal EcoRI site found at 3627
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 502
    Illegal EcoRI site found at 1305
    Illegal EcoRI site found at 1623
    Illegal EcoRI site found at 3466
    Illegal EcoRI site found at 3627
    Illegal XbaI site found at 1638
    Illegal XbaI site found at 3642
    Illegal SpeI site found at 3599
    Illegal SpeI site found at 5184
    Illegal PstI site found at 1438
    Illegal PstI site found at 3613
    Illegal PstI site found at 5198
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal EcoRI site found at 1305
    Illegal EcoRI site found at 1623
    Illegal EcoRI site found at 3466
    Illegal EcoRI site found at 3627
    Illegal XbaI site found at 16
    Illegal XbaI site found at 1638
    Illegal XbaI site found at 3642
    Illegal SpeI site found at 502
    Illegal SpeI site found at 3599
    Illegal SpeI site found at 5184
    Illegal PstI site found at 516
    Illegal PstI site found at 1438
    Illegal PstI site found at 3613
    Illegal PstI site found at 5198
    Illegal AgeI site found at 1023
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 27
    Illegal BsaI site found at 1649
    Illegal BsaI site found at 3652
    Illegal BsaI.rc site found at 1603
    Illegal BsaI.rc site found at 3586
    Illegal BsaI.rc site found at 5171
    Illegal SapI site found at 574


Design Notes

The RBS used in the design were taken from BBa_J61100


Source

ARO8 and KDC genes come from Saccharomyces cerevisiae. AldH gene comes from E. coli.

References